Microarrays within the environmental microbiology toolbox

Microarrays within the environmental microbiology toolbox

Tue, 02/10/2012 - 09:30


About the Presenter

Dr Levente Bodrossy

Levente Bodrossy studied molecular biology and biotechnology at the University of Szeged, Hungary. He did his Ph.D. at the Biological Research Centre of the Hungarian Academy of Sciences with Kornél Kovács in collaboration with Colin Murrell (University of Warwick, UK) on thermophilic methane oxidising bacteria and their biotechnological potential. 

Following his Ph.D. he took a position as lecturer at the Department of Biotechnology of the University of Szeged, where he worked on the biotechnological potential of thermophilic methanotrophs. In 2000 he moved to the Austrian Research Centers where he developed and applied various microarray technologies for microbial ecology and microbial diagnostics.

In 2010 he moved to CSIRO Marine and Atmospheric Research. His current research focuses on the understaning of the interactions between the marine microbiota and the marine environment, including biogeochemical cycles and macrobial ecology. A key step towards this understanding is the development and application of highly affordable molecular methods for the high throughput and high resolution analysis of the composition of marine microbial communities.


Venue

Charles Darwin University
Ellengowan Drive
Building Red 6.1.10
Casuarina NT 0810
Australia

Microbial communities are large and complex, to an extent often challenging human apprehension. An intrinsic challenge of microbial ecology is to assay this complexity in a way that promotes understanding. Every molecular method applied in microbial ecology has advantages and disadvantages, and it is an important task to make the best compromise between these methods in light of the experimental questions raised.

Microarrays enable a reasonably high throughput in terms of sample number while providing a fairly high resolution of detected taxa, genes or gene variants. This combination makes microarrays often an optimal compromise between throughput and resolution in microbial ecology.

The talk will describe the development and validation of a novel amoA microarray, followed by selected tales from nitrifier and methanotroph ecology studies illustrating the potential and limitations of microarray technology in microbial ecology.

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