Analysis of intragenomic variation of the rDNA internal transcribed spacers (ITS) in Halichondrida (Porifera: Demospongiae)

Analysis of intragenomic variation of the rDNA internal transcribed spacers (ITS) in Halichondrida (Porifera: Demospongiae)

Title
Publication TypeJournal Article
Year of Publication2007
AuthorsAlvarez, B, Krishnan, M, Gibb, KS
JournalJournal of the Marine Biological Association of the United Kingdom
Volume87
Issue6
Pagination1599 - 1605
Date Published01/01/07
ISSN0025-3154
Abstract

Three species of sponges, Axinella aruensis, Phakellia sp. and Acanthella cavernosa, were selected to investigate the extent of intragenomic variation (IGV) in the internal transcribed spacers (ITS-1 and ITS-2) of the ribosomal DNA within the order Halichondrida. The IGV was detected in both the spacers and in the three species. At least three non-identical sequences were found in any single individual with the number of polymorphic sites within species ranging from 6 to 77. Uncorrected distance values up to 29% were observed in the ITS-2 of Axinella aruensis. The levels of polymorphism found in this species are the highest reported for Porifera and comparable to those observed in hybrid species of scleractinean corals. Analyses of ITS sequences from multiple clones from individuals of Halichondrida are strongly recommended if these genetic markers are to be used to depict phylogenetic and/or phylogeographic relationships of closely related species and their populations. Data from other nuclear and mitochondrial markers should be used to complement conclusions derived from studies based on ITS sequences.

URLhttp://espace.cdu.edu.au/view/cdu:2645

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