A new grapevine yellows phytoplasma from the Buckland Valley of Victoria, Australia

A new grapevine yellows phytoplasma from the Buckland Valley of Victoria, Australia

Title
Publication TypeJournal Article
Year of Publication2002
AuthorsConstable, F, Whiting, JR, Gibb, KS, Symons, RH
JournalVitis
Volume41
Issue3
Pagination147 - 153
Date Published01/01/02
ISSN0042-7500
Keywords16s ribosomal-rna, aster yellows, australia, classification, differentiation, grapevine yellows, mycoplasmalike organisms, nonribosomal dna, pcr amplification, phytoplasma, sequence, subgroup, x-disease
Abstract

A new phytoplasma detected in grapevines with grapevine yellows disease from the Buckland Valley of Victoria, Australia was characterized. Buckland Valley grapevine yellows phytoplasma (BVGYp) could not be amplified by PCR using primers specific for the stolbur (STOL, 16SrXII) group of phytoplasmas indicating that it was unlikely to be a STOL group phytoplasma. BVGYp was amplified by PCR using primers specific for both the aster yellows (AV, 16Sr I) and STOL phytoplasma groups, indicating that it may be more closely related to the AY group phytoplasmas. Sequence analysis of 16SrRNA gene sequences showed that BVGYp clustered with AY and STOL groups of phytoplasmas. Sequence similarities were determined by pairwise comparisons of the 16S rDNA sequence of BVGYp WAY and STOL group phytoplasmas and BVGYp was more closely related to the AY group phytoplasmas. Although the data indicate BVGYp may form a newAY subgroup, the similarity coefficients between BVGYp and phytoplasmas from the AY, STOL and Mexican periwinkle virescence groups, derived from putative RFLP patterns, were less than 90%, so BVGYp may actually form a new phytoplasma group.

URLhttp://espace.cdu.edu.au/view/cdu:722

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